Composite

Part:BBa_K4160014:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


GEMS receptor construct containing PR3 fused to HA-tag as affinity domain with 31 amino acid linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2441
    Illegal PstI site found at 191
    Illegal PstI site found at 1129
    Illegal PstI site found at 2124
    Illegal PstI site found at 2285
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 760
    Illegal NheI site found at 1498
    Illegal PstI site found at 191
    Illegal PstI site found at 1129
    Illegal PstI site found at 2124
    Illegal PstI site found at 2285
    Illegal NotI site found at 2428
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 760
    Illegal BglII site found at 1602
    Illegal BglII site found at 1788
    Illegal BglII site found at 2052
    Illegal BamHI site found at 64
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 2435
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2441
    Illegal PstI site found at 191
    Illegal PstI site found at 1129
    Illegal PstI site found at 2124
    Illegal PstI site found at 2285
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2441
    Illegal PstI site found at 191
    Illegal PstI site found at 1129
    Illegal PstI site found at 2124
    Illegal PstI site found at 2285
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002 BBa_K4160004, BBa_K1150016, and BBa_K2217015.

References